flowjotm version 7.6.5 Search Results


tm version 7.6.5 windows  (Becton Dickinson)
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Becton Dickinson tm version 7.6.5 windows
Tm Version 7.6.5 Windows, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry data  (Tree Star Inc)
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Tree Star Inc flow cytometry data
RNAi knockdown of ACVRL1 in HMEC-1 cells. (A) The graph illustrates the alteration of ACVRL1 mRNA levels after RNAi using qRT-PCR analysis. Two different primer pairs were used for the analysis of the ACVRL1 gene (see materials and methods). A significant reduction in ACVRL1 mRNA expression was seen after RNAi knockdown. Although significance was not reached, on average ACVRL1 reduction at the protein level was also detected by flow <t>cytometry</t> (B) as well as Western blot analysis (C) following siACVRL1 RNAi. A one-tailed, unpaired Student’s t-test was used. Significant results (p≤0.05) are indicated with an asterisk (*). Experiments were conducted as triplicate (n=3). GAPDH was used as a loading control. ImageJ was used for protein quantification of Western blot results. The image in (A) was created with Affinity Designer 2 (version 2.6.4).
Flow Cytometry Data, supplied by Tree Star Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flowjotm+version+7%2E6%2E5/flowjotm+software/pmc12764187-88-1-13
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flow cytometry data - by Bioz Stars, 2026-09
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an argon ion laser (488 nm)  (Becton Dickinson)
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Becton Dickinson an argon ion laser (488 nm)
RNAi knockdown of ACVRL1 in HMEC-1 cells. (A) The graph illustrates the alteration of ACVRL1 mRNA levels after RNAi using qRT-PCR analysis. Two different primer pairs were used for the analysis of the ACVRL1 gene (see materials and methods). A significant reduction in ACVRL1 mRNA expression was seen after RNAi knockdown. Although significance was not reached, on average ACVRL1 reduction at the protein level was also detected by flow <t>cytometry</t> (B) as well as Western blot analysis (C) following siACVRL1 RNAi. A one-tailed, unpaired Student’s t-test was used. Significant results (p≤0.05) are indicated with an asterisk (*). Experiments were conducted as triplicate (n=3). GAPDH was used as a loading control. ImageJ was used for protein quantification of Western blot results. The image in (A) was created with Affinity Designer 2 (version 2.6.4).
An Argon Ion Laser (488 Nm), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flowjotm+version+7%2E6%2E5/argon+ion+laser/pm37743639-140-45-56
Average 90 stars, based on 1 article reviews
an argon ion laser (488 nm) - by Bioz Stars, 2026-09
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cellquest pro 4.0.1  (Becton Dickinson)
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Becton Dickinson cellquest pro 4.0.1
RNAi knockdown of ACVRL1 in HMEC-1 cells. (A) The graph illustrates the alteration of ACVRL1 mRNA levels after RNAi using qRT-PCR analysis. Two different primer pairs were used for the analysis of the ACVRL1 gene (see materials and methods). A significant reduction in ACVRL1 mRNA expression was seen after RNAi knockdown. Although significance was not reached, on average ACVRL1 reduction at the protein level was also detected by flow <t>cytometry</t> (B) as well as Western blot analysis (C) following siACVRL1 RNAi. A one-tailed, unpaired Student’s t-test was used. Significant results (p≤0.05) are indicated with an asterisk (*). Experiments were conducted as triplicate (n=3). GAPDH was used as a loading control. ImageJ was used for protein quantification of Western blot results. The image in (A) was created with Affinity Designer 2 (version 2.6.4).
Cellquest Pro 4.0.1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flowjotm+version+7%2E6%2E5/cellquest+software/pm37743639-140-53-56
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cellquest pro 4.0.1 - by Bioz Stars, 2026-09
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facscalibur  (Becton Dickinson)
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Becton Dickinson facscalibur
RNAi knockdown of ACVRL1 in HMEC-1 cells. (A) The graph illustrates the alteration of ACVRL1 mRNA levels after RNAi using qRT-PCR analysis. Two different primer pairs were used for the analysis of the ACVRL1 gene (see materials and methods). A significant reduction in ACVRL1 mRNA expression was seen after RNAi knockdown. Although significance was not reached, on average ACVRL1 reduction at the protein level was also detected by flow <t>cytometry</t> (B) as well as Western blot analysis (C) following siACVRL1 RNAi. A one-tailed, unpaired Student’s t-test was used. Significant results (p≤0.05) are indicated with an asterisk (*). Experiments were conducted as triplicate (n=3). GAPDH was used as a loading control. ImageJ was used for protein quantification of Western blot results. The image in (A) was created with Affinity Designer 2 (version 2.6.4).
Facscalibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flowjotm+version+7%2E6%2E5/facscalibur/pm37743639-140-33-36
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Image Search Results


RNAi knockdown of ACVRL1 in HMEC-1 cells. (A) The graph illustrates the alteration of ACVRL1 mRNA levels after RNAi using qRT-PCR analysis. Two different primer pairs were used for the analysis of the ACVRL1 gene (see materials and methods). A significant reduction in ACVRL1 mRNA expression was seen after RNAi knockdown. Although significance was not reached, on average ACVRL1 reduction at the protein level was also detected by flow cytometry (B) as well as Western blot analysis (C) following siACVRL1 RNAi. A one-tailed, unpaired Student’s t-test was used. Significant results (p≤0.05) are indicated with an asterisk (*). Experiments were conducted as triplicate (n=3). GAPDH was used as a loading control. ImageJ was used for protein quantification of Western blot results. The image in (A) was created with Affinity Designer 2 (version 2.6.4).

Journal: In Vivo

Article Title: Modulating ACVRL1 Expression in HMEC1 Cells as a Simplified In Vitro Model for Hereditary Hemorrhagic Telangiectasia (HHT) Type 2 Studies

doi: 10.21873/invivo.14173

Figure Lengend Snippet: RNAi knockdown of ACVRL1 in HMEC-1 cells. (A) The graph illustrates the alteration of ACVRL1 mRNA levels after RNAi using qRT-PCR analysis. Two different primer pairs were used for the analysis of the ACVRL1 gene (see materials and methods). A significant reduction in ACVRL1 mRNA expression was seen after RNAi knockdown. Although significance was not reached, on average ACVRL1 reduction at the protein level was also detected by flow cytometry (B) as well as Western blot analysis (C) following siACVRL1 RNAi. A one-tailed, unpaired Student’s t-test was used. Significant results (p≤0.05) are indicated with an asterisk (*). Experiments were conducted as triplicate (n=3). GAPDH was used as a loading control. ImageJ was used for protein quantification of Western blot results. The image in (A) was created with Affinity Designer 2 (version 2.6.4).

Article Snippet: The flow cytometry data was then analyzed with the FlowJoTM software (version 7.6.5, Tree Star Inc., Ashland, OR, USA).

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Flow Cytometry, Western Blot, One-tailed Test, Control